However, Koch never credited the Hesses for their discovery of bacteriological agar, perhaps because, at the time, he failed to recognize its importance. Even after he received the insight about agar from the Hesses, Koch stuck with gelatine for years. In 1883-84, during his first medical expedition to Egypt and India to investigate cholera, he tried and failed to grow the cholera bacterium on gelatine media in the hot climate of Cairo (despite using a half-open fridge for incubation), only succeeding in the colder winter of Calcutta.
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[3] T. Knoll: “Pattern Dithering” (1999). US Patent No. 6,606,166. ↑。关于这个话题，Line官方版本下载提供了深入分析